Isolation and Identification of Antibiotic Resistant Bacteria among Members of Enterobacteriaceae Isolated from Select Fish Farms in Katsina Metropolis, Nigeria

Published: 2024-12-23
Author(s): Muhammad A. Sa’id, Yusif Abdurrahman, Naziru Abdullahi, Musa AA & Mustapha S. Yauri
Abstract:
Background: Enterobacteriaceae family is a non-spore-forming, facultative anaerobes that include a wide range of gram-negative bacteria found in the gastrointestinal tracts of humans and warm blooded animals.
Objective: The focus of the study was to isolate and identify antibiotic resistance bacteria among the member of Enterobacteriaceae from fish farms within Katsina metropolis. Methods: Total of 100 samples of Clarias gariepinus with an average weight of 78.23g were randomly collected weekly from the fish farms in each of the locations for a period of five weeks. The isolation of the bacteria was carry out through serial dilution method and the isolates identified through gram staining, and biochemical tests, Cefoxitin 30µg), Tetracycline (10µg), Ciprofloxacin (5µg), Erythromycin (15µg), Chloramphenicol (30µg) and Gentamycin (30µg) were used for screening the isolates and Vitek -?2-compact System was uses for identification multi-drug resistance isolates. Results: Different bacterial species were found to be resistant to one or more antibiotic these include Klebsiella Pneumoniae, E.coli, and Proteus mirabilis. A high percentage of resistance to Erythromycin and Chloramphenicol was shown for 96% and 49% respectively of the total isolates.
Conclusion: Conclusively the research highlighted different multi-drug resistance enterobacteriaceae associated with Clarias gariepinus in the fish farms within Katsina metropolis, which of great concern to consumers and public health. This raises the growing concern in searching for alternative treatment.
Unique Contribution: The study highlighted some members of enterobacteriaceae family with anti-biotic resistance against some common antibiotic use in treatment of bacterial diseases in fish farm.
Key Recommendation: Molecular identification will reveal specific resistance mechanism, this help to predicts trend in resistance development and offering data that can inform better management practices.
Keywords: Enterobacteriaceae, Clarias gariepinus, Serial dilution, Multi-drug resistance,
Issue IJSSAR Volume 2, Issue 4, December 2024
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Copyright Copyright © 2024 Muhammad A. Sa’id, Yusif Abdurrahman, Naziru Abdullahi, Musa AA & Mustapha S. Yauri

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Journal Identifiers
eISSN: 3043-4459
pISSN: 3043-4467