International Journal of Sub-Saharan African Research (IJSSAR)

Background: Fusidic acid is one of the most effective antibiotics against methicillin resistant Staphylococcus aureus (MRSA), making it a valuable option for the treatment of S. aureus infections. The present study aimed at evaluation of Minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MBC) of tested virulent S. aureus strain CM05 on fusidic acid
Objective: This study examined actions of Fusidic Acid as an alternative therapeutic option for Staphylococcus aureus Infections in the era of increased antimicrobial resistance. Methodology: Clinical Microbiology strain (CMO5) of S. aureus from Dr Sue Langs’ collection with her approval and Oxford strain of S. aureus for MIC control were obtained and reconfirmed before use in this study between April to December, 2014. Repetition of the experiment on the isolates were carried out through cultural, gram reaction, catalase, agglutination and API web tests. The confirmed isolates were subcultured into Blood agar (BA) (Colombia agar), Brain heart infusion agar (BHI), Muller-Hinton broth & agar (MH) and Trypton soya broth (TSB). All media were from Oxoid, Ltd, Basingstoke Hampshire, and England. Antimicrobial susceptibility testing was performed according to CLSI, 2014 guidelines. Results: The strain showed hemolysis on blood agar, round shaped and size of 2 ?m. Gram reaction showed positive cocci in cluster, catalase and coagulase positive. API identification revealed a 97.8% probability of the isolate being Staphylococcus aureus with biochemical reactions used to obtain 7 unique numbers that was matched with API web software based on colour. The MIC and MBC of fusidic acid against strain CM05 were 0.12 ?g/mL and 0.5 ?g/mL, respectively in relation to oxford strain of 0.12- 0.5 ?g/mL range that was used as control.
Conclusion: The work showed Fusdic acid as an alternative in antibiotic of choice in treating S. aureus related infection and confirmed the identity of our strain for this study which is CM05.
Unique Contribution: This study has offered an alternative means for rising antimicrobial resistance.
Key Recommendation: Further study in DNA, RNA, and cDNA of CMO5 strain with the experiment to be performed more than twice for appropriate results comparison and bridge of information gap in the research area.